IL-8 Protein (AA 28-99) (Biotin)
Quick Overview for IL-8 Protein (AA 28-99) (Biotin) (ABIN6952239)
Target
See all IL-8 (IL8) ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 28-99
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Purification tag / Conjugate
- This IL-8 protein is labelled with Biotin.
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Sequence
- SAKELRCQCI KTYSKPFHPK FIKELRVIES GPHCANTEII VKLSDGRELC LDPKENWVQR VVEKFLKRAE NS
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Characteristics
- Biotininylated Recombinant Human CXCL8 (IL-8)
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Endotoxin Level
- <0.01 EU per 1μg of the protein by the LAL method
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Biological Activity Comment
- EC50 = 0.5-1 nM determined by Migration Assay in cells expressing recombinant CXCR1
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Interleukin 8 (IL8) (AA 28-99) protein (His tag)
IL8 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 8 (IL8) (AA 28-99) (Active) protein (His tag,AVI tag,Biotin)IL8 Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Interleukin 8 (IL8) (AA 26-167) protein (His-IF2DI Tag)IL8 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Interleukin 8 (IL8) (AA 28-99) (Active) proteinIL8 Origin: Human Host: Escherichia coli (E. coli) Recombinant CMA Active
Interleukin 8 (IL8) (AA 1-99) protein (GST tag)IL8 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Interleukin 8 (IL8) (AA 28-99) protein (Fc Tag)IL8 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
Interleukin 8 (IL8) (AA 28-99) (Active) protein (Fc Tag)IL8 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 8 (IL8) (AA 23-101), (full length) protein (His tag)IL8 Origin: Dog Host: Yeast Recombinant > 90 % ELISA
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Spin tube prior to resuspending. Recommended at 100 μg/mL in sterile water
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Storage
- -20 °C,-80 °C
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Storage Comment
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12 months from date of receipt, -20 to -70 °C as supplied.
Suggest to use immediately after reconstitution
1 month at -20 to -70 °C under sterile conditions after reconstitution. -
Expiry Date
- 12 months
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- IL-8 (IL8) (Interleukin 8 (IL8))
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Alternative Name
- CXCL8 (IL-8)
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Background
- Interleukin 8 (IL-8 /CXCL8) is secreted primarily by macrophages and monocytes. It is one of the key mediators for inflammatory responses. IL-8 is a strong chemotractant for neutrophils and monocytes promoting activation of these cells by binding to two cell surface receptors, CXCR1 and CXCR2. It is also a strong angiogenic agent and is considered to play a role in the pathogenesis of bronchiolitis.
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Molecular Weight
- 10.803 kDa
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UniProt
- P10145
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Pathways
- TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy
Target
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