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SNCA Protein (A53T)

Name: SNCA Protein (A53T)
Brand: antibodies-online
SKU: ABIN6952303
Price: 615 USD
Availability: InStock

This Recombinant SNCA protein is produced in Escherichia coli (E. coli).

Primary rat hippocampal neurons show lewy body inclusion formation when treated with A53T mutant Alpha Synuclein Protein Pre-formed Fibrils (ABIN6952303, ABIN6952304 and ABIN6952305) (B) but not when treated with a media control (A). (SNCA Protein (A53T))

Evaluation of a-syn toxicity on primary mouse cortical neurons. (SNCA Protein (A53T))

TEM of A53T alpha synuclein Pre-formed Fibrils (ABIN6952303, ABIN6952304 and ABIN6952305) (SNCA Protein (A53T))

Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures such as those in alpha synuclein fibrils. (SNCA Protein (A53T))

6 images

Catalog No. ABIN6952303

Quick Overview for SNCA Protein (A53T) (ABIN6952303)

Target

See all SNCA Proteins

SNCA (Synuclein, alpha (SNCA))

Protein Type

Recombinant

Biological Activity

Active

Origin

Human

Source

Escherichia coli (E. coli)

Application

SDS-PAGE (SDS), Western Blotting (WB), In vitro Assay (in vitro), In vivo Studies (in vivo)

Protein Characteristics

A53T

Sequence

MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVTTVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA

Specificity

~14.46 kDa

Characteristics

Active Human Recombinant A53T Mutant Alpha Synuclein Protein Preformed Fibrils (Type 1)

Purification

Ion-exchange Purified

Biological Activity Comment

100 μM A53T alpha synuclein protein monomer (SPR-325) seeded with 10 nM A53T alpha synuclein protein PFF (SPR-326) in 25 μM Thioflavin T (PBS pH 7.4, 100 μl reaction volume) generated a fluorescence intensity of 28 000 Relative Fluorescence Units after incubation at 37°C with shaking at 600 rpm for 56 hours. Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a Molecular Devices Gemini XPS microplate reader.
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Expression System

Conjugate

Origin

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SNCA Protein (AA 1-140) (His tag)

Expression System HEK-293 Cells

Conjugate His tag

Origin Human

Price starts at $13,686.36

SNCA Protein (AA 1-140) (Strep Tag)

Expression System Cell-free protein synthesis (CFPS)

Conjugate Strep Tag

Origin Human

Price starts at $20,480.57

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Synuclein, alpha (SNCA) (full length) (Active) protein
SNCA Origin: Mouse Host: Escherichia coli (E. coli) Recombinant SDS, WB, in vitro, in vivo Active

Synuclein, alpha (SNCA) (full length) (Active) protein
SNCA Origin: Human Host: Escherichia coli (E. coli) Recombinant >95% pure using SDS-PAGE analysis. SDS, WB, in vitro, in vivo Active

Synuclein, alpha (SNCA) (AA 1-140) protein (His tag)
custom-made SNCA Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Synuclein, alpha (SNCA) (AA 1-140) protein (His tag)
custom-made SNCA Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Synuclein, alpha (SNCA) protein
SNCA Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE

Synuclein, alpha (SNCA) (AA 1-140) protein (His tag)
SNCA Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA

Synuclein, alpha (SNCA) (active) protein
SNCA Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % pure SDS

Synuclein, alpha (SNCA) (AA 31-140) protein (GST tag)
SNCA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA

Synuclein, alpha (SNCA) (AA 1-140) protein (GST tag)
SNCA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA

Synuclein, alpha (SNCA) (AA 1-139), (partial) protein (GST tag)
SNCA Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % SDS, ELISA

Comment

Certified >95% pure using SDS-PAGE analysis.

Restrictions

For Research Use only
Concentration

Lot specific

Buffer

PBS pH 7.4

Storage

-80 °C
Target

SNCA (Synuclein, alpha (SNCA))

Alternative Name

Alpha Synuclein

Background

Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals. Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus. Functionally, it has been shown to significantly interact with tubulin, and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of the cognitive functions, inactivation may lead to impaired spatial learning and working memory. SNCA fibrillar aggregates represent the major non A-beta component of Alzheimers disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson's disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin. The A53T mutation is a missense point mutation where alanine is replaced by threonine at the 53rd amino acid. This mutation has been linked to early-onset Parkinson's Disease and increased rates of alpha synuclein fibrillization.

Gene ID

6622

NCBI Accession

NP_000336

Pathways

Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Platelet-derived growth Factor Receptor Signaling, Negative Regulation of Transporter Activity, Regulation of long-term Neuronal Synaptic Plasticity