SARS-CoV-2 Spike S1 Protein (RBD) (His tag,AVI tag,Biotin)

Catalog No. ABIN6952456

This Recombinant SARS-CoV-2 Spike S1 protein is expressed in HEK-293 Cells and has been mentioned in 5+ publications.

Quick Overview for SARS-CoV-2 Spike S1 Protein (RBD) (His tag,AVI tag,Biotin) (ABIN6952456)

Target: SARS-CoV-2 Spike S1

Protein Type: Recombinant

Origin: SARS Coronavirus-2 (SARS-CoV-2)

Source: HEK-293 Cells

Purity: > 90 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS.

Product Details

Protein Characteristics: RBD

Purification tag / Conjugate: This SARS-CoV-2 Spike S1 protein is labelled with His tag,AVI tag,Biotin.

Sequence: AA 319-541

Specificity: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Characteristics: Biotinylated 2019-nCoV (COVID-19) S protein RBD, His,Avitag (MALS verified) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Phe 541 (Accession # QHD43416.1). Predicted N-terminus: Arg 319 This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Application Notes: The protein has a calculated MW of 28.7 kDa. The protein migrates as 34-37 kDa under reducing (R) condition due to glycosylation.

Comment:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C,-80 °C

Storage Comment: For long term storage, the product should be stored at lyophilized state at -20°C or lower. This product is stable aftr storage at: -20°C to -70°C for 12 months in lyophilized state, -70°C for 3 months under sterile conditions after reconstitution.

References

Dogan, Kozhaya, Placek, Gunter, Yigit, Hardy, Plassmeyer, Coatney, Lillard, Bukhari, Kleinberg, Hayes, Arditi, Klapper, Merin, Liang, Gupta, Alpan, Unutmaz: "SARS-CoV-2 specific antibody and neutralization assays reveal the wide range of the humoral immune response to virus." in: Communications biology, Vol. 4, Issue 1, pp. 129, (2021) (PubMed).

Bell, Powell, Rodriguez, Cochran, Kim: "Neutralizing antibodies targeting the SARS-CoV-2 receptor binding domain isolated from a naïve human antibody library." in: Protein science : a publication of the Protein Society, Vol. 30, Issue 4, pp. 716-727, (2021) (PubMed).

Prieto-Fernández, Egia-Mendikute, Vila-Vecilla, Bosch, Barreira-Manrique, Lee, García-Del Río, Antoñana-Vildosola, Jiménez-Lasheras, Moreno-Cugnon, Jiménez-Barbero, Berra, Ereño-Orbea, Palazon: "Hypoxia reduces cell attachment of SARS-CoV-2 spike protein by modulating the expression of ACE2, neuropilin-1, syndecan-1 and cellular heparan sulfate." in: Emerging microbes & infections, Vol. 10, Issue 1, pp. 1065-1076, (2021) (PubMed).

Mulligan, Lyke, Kitchin, Absalon, Gurtman, Lockhart, Neuzil, Raabe, Bailey, Swanson, Li, Koury, Kalina, Cooper, Fontes-Garfias, Shi, Türeci, Tompkins, Walsh, Frenck, Falsey, Dormitzer, Gruber, Şahin et al.: "Phase I/II study of COVID-19 RNA vaccine BNT162b1 in adults. ..." in: Nature, Vol. 586, Issue 7830, pp. 589-593, (2020) (PubMed).

Guo, Yao, Yang, Zhang, Qi, Gou, Xi, Liu, Zhang, Cheng, Wang, Rong, Chen, Kong: "Plasma-activated water: An alternative disinfectant for S protein inactivation to prevent SARS-CoV-2 infection." in: Chemical engineering journal (Lausanne, Switzerland : 1996), pp. 127742, (2020) (PubMed).

Target Details

Target: SARS-CoV-2 Spike S1

Target Type: Viral Protein

Background: It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Molecular Weight: 28.2 kDa

Gene ID: 43740568

UniProt: P0DTC2