SARS-CoV Spike Protein (RBD) (His tag)

Catalog No. ABIN6952627

This Recombinant SARS-CoV Spike protein is expressed in HEK-293 Cells and has been mentioned in 1 publication.

Quick Overview for SARS-CoV Spike Protein (RBD) (His tag) (ABIN6952627)

Target: SARS-CoV Spike (SARS-CoV S) (SARS-Coronavirus Spike Protein (SARS-CoV S))

Protein Type: Recombinant

Origin: SARS Coronavirus (SARS-CoV)

Source: HEK-293 Cells

Application: ELISA, SDS-PAGE (SDS)

Purity: > 90 % as determined by SDS-PAGE.
> 95 % as determined by SEC-MALS.

Product Details

Protein Characteristics: RBD

Purification tag / Conjugate: This SARS-CoV Spike protein is labelled with His tag.

Purpose: SARS S protein RBD, His Tag (MALS verified)

Sequence: AA 306-527

Characteristics: SARS S protein RBD, His Tag is expressed from human 293 cells (HEK293). It contains AA Arg 306 - Phe 527 (Accession # AAP13567.1). Predicted N-terminus: Arg 306
This protein carries a polyhistidine tag at the C-terminus.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
This product is stable after storage at:
4-8°C for 12 months in lyophilized state,
-70°C for 3 years under sterile conditions after reconstitution.

References

Longworth, Dittmar: "An antigen microarray protocol for COVID-19 serological analysis." in: STAR protocols, Vol. 2, Issue 3, pp. 100815, (2021) (PubMed).

Target Details

Target: SARS-CoV Spike (SARS-CoV S) (SARS-Coronavirus Spike Protein (SARS-CoV S))

Alternative Name: SARS-Coronavirus Spike Protein

Target Type: Viral Protein

Background: It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Molecular Weight: 26.9 kDa