SARS-CoV Spike Protein (RBD) (His-Avi Tag)
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- Target See all SARS-CoV Spike (SARS-CoV S) Proteins
- SARS-CoV Spike (SARS-CoV S) (SARS-Coronavirus Spike Protein (SARS-CoV S))
- Protein Type
- Recombinant
- Protein Characteristics
- RBD
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Origin
- SARS Coronavirus (SARS-CoV)
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This SARS-CoV Spike protein is labelled with His-Avi Tag.
- Sequence
- Arg306-Phe527
- Purity
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized SARS Spike RBD, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 39.0ng/ml determined by ELISA. See testing image for detail.
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SARS-Coronavirus Spike Protein (SARS-CoV S) (RBD) protein (His tag)
SARS-CoV S Origin: SARS Coronavirus (SARS-CoV) Host: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE.
> 95 % as determined by SEC-MALS. ELISA, SDSSARS-Coronavirus Spike Protein (SARS-CoV S) (RBD) protein (His-Avi Tag,Biotin)SARS-CoV S Origin: SARS Coronavirus (SARS-CoV) Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-Coronavirus Spike Protein (SARS-CoV S) (RBD) protein (mFc Tag)SARS-CoV S Origin: SARS Coronavirus (SARS-CoV) Host: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE.
SARS-Coronavirus Spike Protein (SARS-CoV S) (AA 14-1195) (Active) protein (His tag)SARS-CoV S Origin: SARS Coronavirus (SARS-CoV) Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- SARS-CoV Spike (SARS-CoV S) (SARS-Coronavirus Spike Protein (SARS-CoV S))
- Alternative Name
- SARS-CoV Spike (SARS-CoV S Products)
- Background
- S protein RBD, S glycoprotein RBD, Spike protein RBD,The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
- Molecular Weight
- 27.9 kDa. Due to glycosylation, the protein migrates to 36-46 kDa based on Tris-Bis PAGE result.
- UniProt
- P59594
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