SARS-CoV Spike Protein (AA 14-1195) (His tag)
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- Target See all SARS-CoV Spike (SARS-CoV S) Proteins
- SARS-CoV Spike (SARS-CoV S) (SARS-Coronavirus Spike Protein (SARS-CoV S))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 14-1195
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Origin
- SARS Coronavirus (SARS-CoV)
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This SARS-CoV Spike protein is labelled with His tag.
- Sequence
- AA 14-1195
- Characteristics
- SARS S protein (R667A, K968P, V969P), His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 14 - Pro 1195 (Accession # AAP13567.1(R667A, K968P, V969P). The recombinant protein is expressed with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (K968P, V969P) and alanine substitutions (R667A) are introduced to stabilize the trimeric prefusion state of SARS-CoV S protein and abolish the furin cleavage site, respectively.
- Purity
- >95 % as determined by SDS-PAGE.
- Endotoxin Level
- Less than 1.0 EU per μg by the LAL method.
- Top Product
- Discover our top product SARS-CoV S Protein
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- Application Notes
- MALS verified
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- PBS
- Storage
- -20 °C
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- Target
- SARS-CoV Spike (SARS-CoV S) (SARS-Coronavirus Spike Protein (SARS-CoV S))
- Alternative Name
- SARS S protein (SARS-CoV S Products)
- Target Type
- Viral Protein
- Background
- It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
- Molecular Weight
- 136.3 kDa
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