SARS-CoV-2 Spike S1 Protein (RBD, V367F) (His tag)

Catalog No. ABIN6952653

This Recombinant SARS-CoV-2 Spike S1 protein is expressed in HEK-293 Cells and has been mentioned in 1 publication.

Quick Overview for SARS-CoV-2 Spike S1 Protein (RBD, V367F) (His tag) (ABIN6952653)

Target: SARS-CoV-2 Spike S1

Protein Type: Recombinant

Origin: SARS Coronavirus-2 (SARS-CoV-2)

Source: HEK-293 Cells

Application: SDS-PAGE (SDS), ELISA

Purity: >95 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: RBD, V367F

Purification tag / Conjugate: This SARS-CoV-2 Spike S1 protein is labelled with His tag.

Purpose: SARS-CoV-2 (COVID-19) S protein RBD (V367F), His Tag

Sequence: AA 319-541

Characteristics: SARS-CoV-2 S protein RBD (V367F), His Tag (SPD-S52H4) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Phe 541 (Accession # QHD43416.1 (V367F). Predicted N-terminus: Arg 319
This protein carries a polyhistidine tag at the C-terminus.
The V367F mutation in the RBD of Spike protein shows a higher binding affinity to human ACE2.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
This product is stable after storage at:
4-8°C for 12 months in lyophilized state,
-70°C for 3 years under sterile conditions after reconstitution.

References

Guo, Huang, Zhang, Yao, Zhou, Shen, Shen, Li, Li, Zhang, Chen, Chen, Wu, Fu, Zeng, Feng, Pi, Wang, Zhou, Lu, Li, Fang, Lu, Hu, Wang, Zhang, Gao, Adrian, Wang, Yu, Peng, Gabibov, Min, Wang, Huang et al.: "A SARS-CoV-2 neutralizing antibody with extensive Spike binding coverage and modified for optimal therapeutic outcomes. ..." in: Nature communications, Vol. 12, Issue 1, pp. 2623, (2021) (PubMed).

Target Details

Target: SARS-CoV-2 Spike S1

Target Type: Viral Protein

Background: It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Molecular Weight: 27.0 kDa

Gene ID: 43740568

UniProt: P0DTC2