SARS-CoV-2 Envelope Protein (SARS-CoV-2 E) (His tag,GST tag)

Catalog No. ABIN6952705

Recombinant SARS-CoV-2 Envelope protein expressed in Escherichia coli (E. coli) and has been mentioned in 2+ publications.

Quick Overview for SARS-CoV-2 Envelope Protein (SARS-CoV-2 E) (His tag,GST tag) (ABIN6952705)

Target: SARS-CoV-2 Envelope (SARS-CoV-2 E)

Protein Type: Recombinant

Origin: SARS Coronavirus-2 (SARS-CoV-2)

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: >90 % as determined by SDS-PAGE.

Product Details

Purification tag / Conjugate: This SARS-CoV-2 Envelope protein is labelled with His tag,GST tag.

Purpose: SARS-CoV-2 (COVID-19) Envelope protein, His Tag

Sequence: AA 1-75

Characteristics: SARS-CoV-2 Envelope protein, GST,His Tag (ENN-C5128) is expressed from E.coli cells. It contains AA Met 1 - Val 75 (Accession # QHU79206.1). Predicted N-terminus: Met
This protein carries a GST tag at the N-terminus and a polyhistidine tag at the C-terminus.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20 mM Tris, 5 mM EDTA, Arginine, pH 8.0

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For long term storage, the product should be stored at lyophilized state at -20°C or lower.

References

Qian, Lei, Patel, Lee, Monaghan-Nichols, Xin, Qiu, Fu: "Direct activation of endothelial cells by SARS-CoV-2 nucleocapsid protein is blocked by Simvastatin." in: bioRxiv : the preprint server for biology, (2021) (PubMed).

Longworth, Dittmar: "An antigen microarray protocol for COVID-19 serological analysis." in: STAR protocols, Vol. 2, Issue 3, pp. 100815, (2021) (PubMed).

Target Details

Target: SARS-CoV-2 Envelope (SARS-CoV-2 E)

Alternative Name: SARS-CoV-2 Envelope Protein

Target Type: Viral Protein

Background: The SARS-CoV-2 is composed of a double-layered lipid envelope, including Spike glycoprotein (S), Envelope protein (E), Membrane glycoprotein (M), and Nucleocapsid protein (Nucleocapsid protein, N). Among them, The amino acid sequence of the SARS-CoV-2 envelope protein is 95 % identical of the SARS envelope protein.

Molecular Weight: 36.8 kDa