ACE2 Protein (AA 18-740) (His-Avi Tag)

Catalog No. ABIN6952743

This Recombinant ACE2 protein is produced in HEK-293 Cells.

Quick Overview for ACE2 Protein (AA 18-740) (His-Avi Tag) (ABIN6952743)

Target: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Application: SDS-PAGE (SDS), Functional Studies (Func)

Purity: > 95 % by SDS-PAGE.

Product Details

Protein Characteristics: AA 18-740

Purification tag / Conjugate: This ACE2 protein is labelled with His-Avi Tag.

Sequence: QSTIEEQAKT FLDKFNHEAE DLFYQSSLAS WNYNTNITEE NVQNMNNAGD KWSAFLKEQS TLAQMYPLQE IQNLTVKLQL QALQQNGSSV LSEDKSKRLN TILNTMSTIY STGKVCNPDN PQECLLLEPG LNEIMANSLD YNERLWAWES WRSEVGKQLR PLYEEYVVLK NEMARANHYE DYGDYWRGDY EVNGVDGYDY SRGQLIEDVE HTFEEIKPLY EHLHAYVRAK LMNAYPSYIS PIGCLPAHLL GDMWGRFWTN LYSLTVPFGQ KPNIDVTDAM VDQAWDAQRI FKEAEKFFVS VGLPNMTQGF WENSMLTDPG NVQKAVCHPT AWDLGKGDFR ILMCTKVTMD DFLTAHHEMG HIQYDMAYAA QPFLLRNGAN EGFHEAVGEI MSLSAATPKH LKSIGLLSPD FQEDNETEIN FLLKQALTIV GTLPFTYMLE KWRWMVFKGE IPKDQWMKKW WEMKREIVGV VEPVPHDETY CDPASLFHVS NDYSFIRYYT RTLYQFQFQE ALCQAAKHEG PLHKCDISNS TEAGQKLFNM LRLGKSEPWT LALENVVGAK NMNVRPLLNY FEPLFTWLKD QNKNSFVGWS TDWSPYADQS IKVRISLKSA LGDKAYEWND NEMYLFRSSV AYAMRQYFLK VKNQMILFGE EDVRVANLKP RISFNFFVTA PKNVSDIIPR TEVEKAIRMS RSRINDAFRL NDNSLEFLGI QPTLGPPNQP PVS

Specificity: Gln18-Ser740

Characteristics: Recombinant Human ACE2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln18-Ser740) of human ACE2 (Accession #Q9BYF1) fused with a 6xHis,Avi tag at the C-terminus.

Sterility: 0.22 μm filtered

Endotoxin Level: < 1.0 EU/μg of the protein by LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized Human ACE2 at 2 μg/mL (100μL/well) can bind SARS-CoV-2 Spike RBD with a linear range of 0.03-4.9 ng/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. or Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80 °C for long term.After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.|or This product is stable at ≤ -70°C for up to 6 months from the date of receipt. |For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.

Target Details

Target: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Alternative Name: ACE2

Background: ACE2, ACEH, angiotensin-converting enzyme 2,ACEH

Gene ID: 59272

UniProt: Q9BYF1

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour