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SARS-CoV-2 Spike (Super Stable Trimer) protein (His tag) Protein

Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS. ELISA
Pubmed (2 references)
Catalog No. ABIN6953302
$495.00
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  • Target See all SARS-CoV-2 Spike Proteins
    SARS-CoV-2 Spike
    Protein Type
    Recombinant
    Protein Characteristics
    Super Stable Trimer
    Origin
    • 349
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    SARS Coronavirus-2 (SARS-CoV-2)
    Source
    • 309
    • 31
    • 7
    • 2
    • 2
    • 1
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This SARS-CoV-2 Spike protein is labelled with His tag.
    Application
    ELISA
    Purpose
    SARS-CoV-2 S protein, His Tag, Super stable trimer (MALS & NS-EM verified)
    Sequence
    AA 16-1213
    Characteristics
    SARS-CoV-2 S protein, His Tag, Super stable trimer is the ectodomain of SARS-CoV-2 S protein which contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus.
    Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
    Purity
    > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Less than 1.0 EU per μg by the LAL method.
  • Application Notes
    This product is specifically designed for antibody drug development.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    PBS
    Storage
    -20 °C,-80 °C
    Storage Comment
    For long term storage, the product should be stored at lyophilized state at -20°C or lower.
    This product is stable after storage at:
    -20°C to -70°C for 12 months in lyophilized state
    70°C for 3 months under sterile conditions after reconstitution.
    Expiry Date
    12 months
  • Yokoyama, Ichiki: "Nano-size dependence in the adsorption by the SARS-CoV-2 spike protein over gold colloid." in: Colloids and surfaces. A, Physicochemical and engineering aspects, Vol. 615, pp. 126275, (2021) (PubMed).

    Wei, Wan, Yan, Wang, Zhang, Yang, Zhang, Fan, Li, Deng, Sun, Gong, Yang, Wang, Wang, Li, Yang, Li, Zhang, Wang, Du, Zong, Yin, Zhang, Wang, Peng, Lin, Feng, Qin, Chen, Gao, Zhang, Cao, Zhong: "HDL-scavenger receptor B type 1 facilitates SARS-CoV-2 entry." in: Nature metabolism, Vol. 2, Issue 12, pp. 1391-1400, (2020) (PubMed).

  • Target
    SARS-CoV-2 Spike
    Abstract
    SARS-CoV-2 Spike Products
    Synonyms
    E2, E2 glycoprotein precursor, Surface Glycoprotein, S
    Target Type
    Viral Protein
    Background
    It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
    Molecular Weight
    138.0 kDa
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