SARS-CoV-2 Spike Protein (N501Y, RBD) (His tag)

Catalog No. ABIN7274385

Product Details

Target: SARS-CoV-2 Spike

Protein Type: Recombinant

Protein Characteristics: N501Y, RBD

Origin: SARS Coronavirus-2 (SARS-CoV-2)

Source: HEK-293 Cells

Purification tag / Conjugate: This SARS-CoV-2 Spike protein is labelled with His tag.

Purpose: SARS-COV-2 Spike RBD (N501Y) Protein

Sequence: Arg319-Phe541 (N501Y)

Characteristics: Recombinant SARS-COV-2 Spike RBD (N501Y) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg319-Phe541(N501Y).

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized SARS-COV-2 Spike RBD (N501Y) , His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 12.7ng/ml determined by ELISA. The affinity constant of 1.74nM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: SARS-CoV-2 Spike

Abstract: SARS-CoV-2 Spike Products

Target Type: Viral Protein

Background: The ongoing coronavirus disease 2019 (COVID-19) pandemic has prioritized the development of small-animal models for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The resulting mouse-adapted strain at passage 6 (called MASCp6) showed increased infectivity in mouse lung and led to interstitial pneumonia and inflammatory responses in both young and aged mice after intranasal inoculation. Deep sequencing revealed a panel of adaptive mutations potentially associated with the increased virulence. In particular, the N501Y mutation is located at the receptor binding domain (RBD) of the spike protein.

Molecular Weight: 26.2 kDa. Due to glycosylation, the protein migrates to 36-40 kDa based on Tris-Bis PAGE result.