IL3RA Protein (AA 19-305) (His tag,Fc Tag)

Catalog No. ABIN6961076

Recombinant IL3RA protein expressed in HEK-293 Cells.

Quick Overview for IL3RA Protein (AA 19-305) (His tag,Fc Tag) (ABIN6961076)

Target: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Screening Assay (ScA), Immunoassay (IA), Affinity Measurement (AM), Antibody Drug Assay (ADA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 19-305

Purification tag / Conjugate: This IL3RA protein is labelled with His tag,Fc Tag.

Purpose: Recombinant human CD123 protein with C-terminal human Fc and 6xHis tag

Specificity: CD123(Thr19-Arg305) hFc(Glu99-Ala330) 6xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Alternative Name: CD123

Background: IL3R,IL3RA,IL-3Ra,IL-3R-alpha,IL3RAY,IL3RX,IL3RY,CD123 antigen,CD123,hIL3Ra,hIL-3Ra,MGC34174,IL-3 R alpha
Interleukin 3 receptor alpha (low affinity) (IL3RA), also known as CD123 (Cluster of Differentiation 123) is a 70-kD glycoprotein member of the hematopoietin receptor superfamily. This protein associates with a beta subunit common to the receptors for IL-5 and granulocyte-macrophage colony-stimulating factor (GM-CSF) to form a high-affinity receptor for IL-3. The interleukin-3 receptor a chain (CD123) has been identified as a potential immunotherapeutic target because it is overexpressed in AML compared with normal hematopoietic stem cells

Molecular Weight: predicted molecular mass of 80-90 kDa after removal of the signal peptide.

Gene ID: 3563

UniProt: P26951

Pathways: JAK-STAT Signaling