FLT3 Protein (AA 27-543) (His tag,Fc Tag)

Catalog No. ABIN6961080

This Recombinant FLT3 protein is produced in HEK-293 Cells.

Quick Overview for FLT3 Protein (AA 27-543) (His tag,Fc Tag) (ABIN6961080)

Target: FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 27-543

Purification tag / Conjugate: This FLT3 protein is labelled with His tag,Fc Tag.

Purpose: Recombinant human FLT3 protein with C-terminal human Fc and 6xHis tag

Specificity: FLT3(Asn27-Ser543) hFc(Glu99-Ala330) 6xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))

Alternative Name: FLT3

Background: Flt-3,Flk-2,STK-1,CD135,FLK2,FLT-3
Flt-3(Receptor-type tyrosine-protein kinase FLT3) is also known as FLK-2(Fetal liver kinase-2), STK-1(Stem cell tyrosine kinase 1), CD135. FLT3 is a cytokine receptor which belongs to the receptor tyrosine kinase class III. Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.

Molecular Weight: predicted molecular mass of 125-130 kDa after removal of the signal peptide.

Gene ID: 2322

UniProt: P36888

Pathways: RTK Signaling