ICOS Protein (AA 21-140) (His tag,mFc Tag)

Catalog No. ABIN6961099

This Recombinant ICOS protein is expressed in HEK-293 Cells.

Quick Overview for ICOS Protein (AA 21-140) (His tag,mFc Tag) (ABIN6961099)

Target: ICOS (Inducible T-Cell Co-Stimulator (ICOS))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Screening Assay (ScA), Immunoassay (IA), Affinity Measurement (AM), Antibody Drug Assay (ADA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 21-140

Purification tag / Conjugate: This ICOS protein is labelled with His tag,mFc Tag.

Purpose: Recombinant human ICOS protein with C-terminal mouse Fc and 6xHis tag

Specificity: ICOS(Glu21-Lys140) mFc(Pro99-Lys330) 6xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: ICOS (Inducible T-Cell Co-Stimulator (ICOS))

Alternative Name: ICOS

Background: ICOS,CD278,AILIM,Inducible T-cell costimulator
The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation.

Molecular Weight: predicted molecular mass of 41.1 kDa after removal of the signal peptide.

Gene ID: 29851

UniProt: Q9Y6W8

Pathways: Cancer Immune Checkpoints