Mesothelin Protein (MSLN) (AA 296-580) (His tag,mFc Tag)

Catalog No. ABIN6961104

This Recombinant Mesothelin protein is produced in HEK-293 Cells.

Quick Overview for Mesothelin Protein (MSLN) (AA 296-580) (His tag,mFc Tag) (ABIN6961104)

Target: Mesothelin (MSLN)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Immunoassay (IA), Antibody Drug Assay (ADA), Affinity Measurement (AM), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 296-580

Purification tag / Conjugate: This Mesothelin protein is labelled with His tag,mFc Tag.

Purpose: Recombinant human Mesothelin(296-580) protein with C-terminal mouse Fc and 6xHis tag

Specificity: Mesothelin(Glu296-Gly580) mFc(Pro99-Lys330) 6xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: Mesothelin (MSLN)

Alternative Name: Mesothelin

Background: MSLN,Mesothelin,MPF
This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.

Molecular Weight: predicted molecular mass of 59.3 kDa after removal of the signal peptide.

Gene ID: 10232

UniProt: Q13421

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process