ICAM1 Protein (AA 28-480) (mFc Tag)
Quick Overview for ICAM1 Protein (AA 28-480) (mFc Tag) (ABIN6961122)
Target
See all ICAM1 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 28-480
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Purification tag / Conjugate
- This ICAM1 protein is labelled with mFc Tag.
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Purpose
- Recombinant human ICAM-1 protein with C-terminal mouse Fc tag
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Specificity
- ICAM-1(Gln28-Glu480) mFc(Pro99-Lys330)
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Characteristics
- Extracellular Domain Protein
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Purification
- Purified from cell culture supernatant by affinity chromatography
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Intercellular Adhesion Molecule 1 (ICAM1) (AA 28-480) (Active) protein (His tag)
ICAM1 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Intercellular Adhesion Molecule 1 (ICAM1) (AA 1-532) protein (GST tag)ICAM1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Intercellular Adhesion Molecule 1 (ICAM1) (Active) protein (His tag)ICAM1 Origin: Mouse Host: HEK-293 Cells Recombinant > 96 % as determined by SDS-PAGE Active
Intercellular Adhesion Molecule 1 (ICAM1) (Active) protein (His tag,Fc Tag)ICAM1 Origin: Mouse Host: HEK-293 Cells Recombinant > 96 % as determined by SDS-PAGE Active
Intercellular Adhesion Molecule 1 (ICAM1) (Active) protein (His tag)ICAM1 Origin: Rat Host: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE Active
Intercellular Adhesion Molecule 1 (ICAM1) (AA 26-272) protein (His-IF2DI Tag)ICAM1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Intercellular Adhesion Molecule 1 (ICAM1) (AA 28-485) protein (His tag)ICAM1 Origin: Mouse Host: Mammalian Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
Intercellular Adhesion Molecule 1 (ICAM1) (AA 28-480), (partial) protein (GST tag)ICAM1 Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
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Application Notes
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Extracellular Domain Proteins (ECD) can be used as:
- Immunogens for antibody drug development
- Reagents used for CAR-T positive cell monitoring
- Reagents for antibody screening and functional testing
- Reagents for antibody affinity measurement
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Comment
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The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Please see Certificate of Analysis for specific instructions of reconstitution.
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Buffer
- Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
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Expiry Date
- 12 months
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- ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))
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Alternative Name
- ICAM-1
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Background
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ICAM1,BB2,CD54,P3.58
This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. -
Molecular Weight
- predicted molecular mass of 75.7 kDa after removal of the signal peptide.The apparent molecular mass of ICAM-1-mFc is 90-120 kDa due to glycosylation.
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Gene ID
- 3383
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UniProt
- P05362
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Pathways
- Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling
Target
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