MUC1 Protein (AA 1036-1156) (His tag)

Catalog No. ABIN6964084

This Recombinant MUC1 protein is expressed in HEK-293 Cells.

Quick Overview for MUC1 Protein (AA 1036-1156) (His tag) (ABIN6964084)

Target: MUC1 (Mucin 1 (MUC1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Immunoassay (IA), Screening Assay (ScA), Antibody Drug Assay (ADA), Affinity Measurement (AM)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 1036-1156

Purification tag / Conjugate: This MUC1 protein is labelled with His tag.

Purpose: Recombinant Human MUC1 Protein with C-terminal 6xHis tag

Specificity: MUC1(Leu1036-Val1156) 6xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: MUC1 (Mucin 1 (MUC1))

Alternative Name: MUC1

Background: ADMCKD,ADMCKD1,CA 15-3,CD227,EMA,H23AG,KL-6,MAM6,MCD,MCKD,MCKD1,MUC-1,MUC-1/SEC,MUC-1/X,MUC1/ZD,PEM,PEMT,PUM
This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.

Molecular Weight: predicted molecular mass of 14.4 kDa after removal of the signal peptide.

Gene ID: 4582

UniProt: P15941

Pathways: Negative Regulation of intrinsic apoptotic Signaling