MICB Protein (AA 23-309) (His tag)

Catalog No. ABIN6964108

This Recombinant MICB protein is expressed in HEK-293 Cells.

Quick Overview for MICB Protein (AA 23-309) (His tag) (ABIN6964108)

Target: MICB (MHC Class I Polypeptide-Related Sequence B (MICB))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 23-309

Purification tag / Conjugate: This MICB protein is labelled with His tag.

Purpose: Recombinant Human MICB Protein with C-terminal 6xHis tag

Specificity: MICB(Ala23-Asp309) 6xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: MICB (MHC Class I Polypeptide-Related Sequence B (MICB))

Alternative Name: MICB

Background: MIC-B,PERB11.2
This gene encodes a heavily glycosylated protein which is a ligand for the NKG2D type II receptor. Binding of the ligand activates the cytolytic response of natural killer (NK) cells, CD8 alphabeta T cells, and gammadelta T cells which express the receptor. This protein is stress-induced and is similar to MHC class I molecules, however, it does not associate with beta-2-microglobulin or bind peptides. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Molecular Weight: predicted molecular mass of 33.5 kDa after removal of the signal peptide.The apparent molecular mass of MICB-His is 35-55 kDa due to glycosylation.

Gene ID: 4277

UniProt: Q29980

Pathways: Human Leukocyte Antigen (HLA) in Adaptive Immune Response