IL12RB1 Protein (AA 24-540) (His tag)

Catalog No. ABIN6964200

This Recombinant IL12RB1 protein is produced in HEK-293 Cells.

Quick Overview for IL12RB1 Protein (AA 24-540) (His tag) (ABIN6964200)

Target: IL12RB1 (Interleukin 12 Receptor beta 1 (IL12RB1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Screening Assay (ScA), Immunoassay (IA), Affinity Measurement (AM), Antibody Drug Assay (ADA)

Purity: The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 24-540

Purification tag / Conjugate: This IL12RB1 protein is labelled with His tag.

Purpose: Recombinant Human IL12RB1 Protein with C-terminal 6xHis tag

Specificity: IL12RB1(Cys24-Glu540) 6xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: IL12RB1 (Interleukin 12 Receptor beta 1 (IL12RB1))

Alternative Name: IL12RB1

Background: CD212,IL-12R-BETA1,IL12RB,IMD30
The protein encoded by this gene is a type I transmembrane protein that belongs to the hemopoietin receptor superfamily. This protein binds to interleukine 12 (IL12) with a low affinity, and is thought to be a part of IL12 receptor complex. This protein forms a disulfide-linked oligomer, which is required for its IL12 binding activity. The coexpression of this and IL12RB2 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. Mutations in this gene impair the development of interleukin-17-producing T lymphocytes and result in increased susceptibility to mycobacterial and Salmonella infections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Molecular Weight: predicted molecular mass of 57.9 kDa after removal of the signal peptide. The apparent molecular mass of IL12RB1-His is 70-100 kDa due to glycosylation.

UniProt: P42701

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation