IL12RB1 Protein (AA 24-540) (Fc Tag,AVI tag,Biotin)

Catalog No. ABIN6731329

Product Details

Target: IL12RB1 (Interleukin 12 Receptor beta 1 (IL12RB1))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 24-540

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This IL12RB1 protein is labelled with Fc Tag,AVI tag,Biotin.

Sequence: AA 24-540

Specificity: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Purity: >95 % as determined by reduced SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Comment:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Tris with Glycine, Arginine and NaCl,  pH 7.5

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Target Details

Target: IL12RB1 (Interleukin 12 Receptor beta 1 (IL12RB1))

Alternative Name: IL-12 R beta 1 (IL12RB1 Products)

Synonyms: Il12rb Protein, IL12RB1 Protein, IL-12R Protein, CD212 Protein, IL-12R-BETA1 Protein, IL12RB Protein, IL-12R[b] Protein, interleukin 12 receptor subunit beta 1 Protein, interleukin 12 receptor, beta 1 Protein, Il12rb1 Protein, IL12RB1 Protein

Background: The human IL-12 R subunit is a member of the cytokine receptor superfamily and the functional high-affinity IL-12R is composed of at least two beta-type cytokine receptor subunits, each independently exhibiting a low affinity for IL-12. IL-12 R beta 1 (Interleukin-12 receptor subunit beta-1) is also known as IL-12RB1, CD212. Functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.

Molecular Weight: 85.3 kDa

NCBI Accession: NP_005526

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation