BRD4 Protein (AA 1-1362) (Fc Tag)

Catalog No. ABIN6964231

This Recombinant BRD4 protein is expressed in HEK-293 Cells.

Quick Overview for BRD4 Protein (AA 1-1362) (Fc Tag) (ABIN6964231)

Target: BRD4 (Bromodomain Containing 4 (BRD4))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Screening Assay (ScA), Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 1-1362

Purification tag / Conjugate: This BRD4 protein is labelled with Fc Tag.

Purpose: Recombinant human BRD4 protein with C-terminal human Fc tag

Specificity: BRD4(Met1-Phe1362) hFc(Glu99-Ala330)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: BRD4 (Bromodomain Containing 4 (BRD4))

Alternative Name: BRD4

Background: CAP,HUNK1,HUNKI,MCAP
The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15,19)(q13,p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008]

Molecular Weight: predicted molecular mass of 178.4 kDa after removal of the signal peptide.

UniProt: O60885

Pathways: Chromatin Binding, SARS-CoV-2 Protein Interactome