BRD4 Protein
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- Target See all BRD4 Proteins
- BRD4 (Bromodomain Containing 4 (BRD4))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purpose
- Recombinant Human BRD4 (N-10His-Flag)
- Sequence
- Glu49-Glu460
- Characteristics
- Recombinant Human Bromodomain-containing protein 4 is produced by our E.coli expression system and the target gene encoding Glu49-Glu460 is expressed with a 10His, Flag tag at the N-terminus.
- Purity
- >95 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product BRD4 Protein
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Bromodomain Containing 4 (BRD4) (AA 49-170) protein (His tag)
BRD4 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Bromodomain Containing 4 (BRD4) (AA 1-1362) protein (Strep Tag)Crystallography grade BRD4 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Bromodomain Containing 4 (BRD4) (AA 1-1400) protein (Strep Tag)Crystallography grade BRD4 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Bromodomain Containing 4 (BRD4) (AA 44-168) protein (His tag,DYKDDDDK Tag)BRD4 Origin: Human Host: Escherichia coli (E. coli) Recombinant Bind, ScA
Bromodomain Containing 4 (BRD4) (AA 333-460) protein (His tag,DYKDDDDK Tag)BRD4 Origin: Human Host: Escherichia coli (E. coli) Recombinant Bind, ScA
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- Restrictions
- For Research Use only
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- Format
- Frozen, Liquid
- Buffer
- Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 200 mM NaCl, 1 mM DTT, 10 % Glycerol, pH 7.5.
- Storage
- -20 °C
- Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- Target
- BRD4 (Bromodomain Containing 4 (BRD4))
- Alternative Name
- BRD4 (BRD4 Products)
- Synonyms
- brd4-b Protein, BRD4 Protein, fb18g03 Protein, wu:fb18g03 Protein, CAP Protein, HUNK1 Protein, HUNKI Protein, MCAP Protein, Brd5 Protein, WI-11513 Protein, bromodomain containing 4 L homeolog Protein, bromodomain containing 4 Protein, bromodomain-containing protein 4 Protein, brd4.L Protein, BRD4 Protein, brd4 Protein, LOC100409351 Protein, Brd4 Protein
- Background
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Background: Bromodomain-containing protein 4 (BRD4) is a member of the BET class chromatin reader proteins that bind acetylated histones and play a key role in transcriptional regulation and transmission of epigenetic memory. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. BRD bromodomains serve as recognition motifs for acetylated lysine residues on histones, while the NET domain may function by promoting phosphorylation of the C-terminal domain (CTD) of RNA Polymerase II. Some specific inhibitors of BRD4 that prevent binding to acetylated histones by binding Asn-140 and Asn-433 are promising therapeutic molecules for the treatment of leukemias. BRD4 is a potential therapeutic target in many diseases including breast cancer, AML, multiple myeloma, colon cancer and others.
Synonym: HUNK1, HUNK1bromodomain-containing protein 4, HUNKI, MCAP
- Molecular Weight
- 49 kDa
- UniProt
- O60885
- Pathways
- Chromatin Binding, SARS-CoV-2 Protein Interactome
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