PRAME Protein (Fc Tag)
Quick Overview for PRAME Protein (Fc Tag) (ABIN6964312)
Target
See all PRAME ProteinsProtein Type
Origin
Source
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Purification tag / Conjugate
- This PRAME protein is labelled with Fc Tag.
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Sequence
- PRAME (Met1-Asn509)+hFc (Glu99-Ala330)
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Characteristics
- Tagged Protein: C-Human Fc tag
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Purification
- affinity purification
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Preferentially Expressed Antigen in Melanoma (PRAME) (AA 1-509) protein (His tag)
custom-made PRAME Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Preferentially Expressed Antigen in Melanoma (PRAME) (AA 1-509) protein (GST tag)PRAME Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Preferentially Expressed Antigen in Melanoma (PRAME) (AA 321-420) protein (His-IF2DI Tag)PRAME Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Preferentially Expressed Antigen in Melanoma (PRAME) (Transcript Variant 4) protein (Myc-DYKDDDDK Tag)PRAME Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Preferentially Expressed Antigen in Melanoma (PRAME) (AA 1-509), (full length) protein (His tag)PRAME Origin: Human Host: Yeast Recombinant > 90 % SDS
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute with deionized water
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Buffer
- Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
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Preservative
- Without preservative
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Storage
- -80 °C
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Storage Comment
- Store at -80°C for 12 months (Avoid repeated freezing and thawing)
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Expiry Date
- 12 months
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- PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))
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Alternative Name
- PRAME
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Background
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Synonymes: CT130, MAPE, OIP-4, OIP4
Description: This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014] -
Molecular Weight
- 81.51 kDa
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UniProt
- P78395
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Pathways
- Retinoic Acid Receptor Signaling Pathway, Nuclear Hormone Receptor Binding
Target
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