SLAMF8 Protein (AA 23-233) (His tag,AVI tag,Biotin)

Catalog No. ABIN6972956

This Recombinant SLAMF8 protein is expressed in HEK-293 Cells.

Quick Overview for SLAMF8 Protein (AA 23-233) (His tag,AVI tag,Biotin) (ABIN6972956)

Target: SLAMF8 (SLAM Family Member 8 (SLAMF8))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: >85 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 23-233

Purification tag / Conjugate: This SLAMF8 protein is labelled with His tag,AVI tag,Biotin.

Specificity: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Characteristics: Biotinylated Human BLAME / SLAMF8 Protein, His,Avitag™

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Comment:

Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 50 mM Sodium Citrate, 150 mM NaCl, pH 5.5

Storage: -20 °C

Target Details

Target: SLAMF8 (SLAM Family Member 8 (SLAMF8))

Alternative Name: BLAME

Background: BLAME (B-lymphocyte activator macrophage expressed) is also known as SLAM family member 8(SLAMF8), CD353. BLAME is a cell surface receptor that is expressed upon activation of macrophages (Ms) by IFN-γ or bacteria, is a negative regulator of ROS in response to Gram+ and Gram- bacteria. May play a role in B-lineage commitment and/or modulation of signaling through the B-cell receptor. SLAMF8 is a costimulatory molecule that affects the activation of macrophages in inflammation and in immunosuppression and inflammation response to glioma cells could aid immunotherapy for glioma.

Molecular Weight: 27.1 kDa

NCBI Accession: NP_064510