BRAF Protein (AA 457-717) (His tag)

Catalog No. ABIN7408507

This Recombinant BRAF protein is produced in Escherichia coli (E. coli).

Quick Overview for BRAF Protein (AA 457-717) (His tag) (ABIN7408507)

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), SDS-PAGE (SDS), Immunogen (Imm), Positive Control (PC)

Purity: > 95 %

Product Details

Protein Characteristics: AA 457-717

Purification tag / Conjugate: This BRAF protein is labelled with His tag.

Sequence: Ile457-Leu717

Characteristics: Recombinant B-Raf Proto Oncogene Serine/Threonine Protein Kinase (BRAF), Prokaryotic expression, Ile457-Leu717, N-terminal His Tag

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Isoelectric Point:9.5

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Expiry Date: 12 months

Target Details

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Alternative Name: B-Raf Proto Oncogene Serine/Threonine Protein Kinase

Background: B-raf 1, BRAF1, RAFB1, V-Raf Murine Sarcoma Viral Oncogene Homolog B1

Molecular Weight: 33kDa

UniProt: P15056

Pathways: MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy