IgM Protein (His tag,SUMO Tag)

Catalog No. ABIN7410937

Recombinant IgM protein expressed in Escherichia coli (E. coli).

Quick Overview for IgM Protein (His tag,SUMO Tag) (ABIN7410937)

Target: IgM

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Western Blotting (WB), Immunogen (Imm), Positive Control (PC)

Purity: > 95 %

Product Details

Purification tag / Conjugate: This IgM protein is labelled with His tag,SUMO Tag.

Purpose: Recombinant Immunoglobulin M (IgM)

Sequence: Ser1-Tyr454

Characteristics: Recombinant Immunoglobulin M (IgM), Prokaryotic expression, Two N-terminal Tags, His-tag and SUMO-tag

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

STABILITY: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Isoelectric Point: 7

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in PBS or others.

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), Other preservative, ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Expiry Date: 12 months

Target Details

Target: IgM

Alternative Name: Immunoglobulin M

Target Type: Antibody

Background: IGHM, Immunoglobulin Heavy Constant Mu, Ig mu chain C region

Molecular Weight: 65kDa

UniProt: P01872