RAC1 Protein (His tag)
Quick Overview for RAC1 Protein (His tag) (ABIN7386275)
Target
Protein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This RAC1 protein is labelled with His tag.
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Purpose
- Recombinant Ras Related C3 Botulinum Toxin Substrate 1 (Rac1)
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Sequence
- Met1-Cys189
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Characteristics
- Recombinant Ras Related C3 Botulinum Toxin Substrate 1 (Rac1), Prokaryotic expression, , N-terminal His Tag
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Endotoxin Level
- <1.0EU per 1μg (determined by the LAL method)
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RAC1 (AA 1-192) protein (His tag)
Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
RAC1 (AA 1-192) protein (GST tag)Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
RAC1 protein (Myc-DYKDDDDK Tag)Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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STABILITY: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Isoelectric Point: 8.8
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute in 10 mM PBS ( pH 7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
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Buffer
- PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.
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Preservative
- Dithiothreitol (DTT), ProClin
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Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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Storage
- 4 °C,-80 °C
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Storage Comment
- Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
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Expiry Date
- 12 months
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- RAC1
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Alternative Name
- Ras Related C3 Botulinum Toxin Substrate 1
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Background
- TC-25, p21-Rac1, MIG5, Cell migration-inducing gene 5 protein, Ras-like protein TC25, p21-Rac1
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Molecular Weight
- 25kDa
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UniProt
- Q6RUV5
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Pathways
- WNT Signaling, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Thromboxane A2 Receptor Signaling, VEGF Signaling
Target
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