RPA1 Protein (AA 18-403) (His tag,GST tag)

Catalog No. ABIN7408948

This Recombinant RPA1 protein is expressed in Escherichia coli (E. coli).

Quick Overview for RPA1 Protein (AA 18-403) (His tag,GST tag) (ABIN7408948)

Target: RPA1 (Replication Protein A1, 70kDa (RPA1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Western Blotting (WB), Immunogen (Imm), Positive Control (PC)

Purity: > 95 %

Product Details

Protein Characteristics: AA 18-403

Purification tag / Conjugate: This RPA1 protein is labelled with His tag,GST tag.

Purpose: Recombinant Replication Protein A1 (RPA1)

Sequence: Asp18-Pro403

Characteristics: Recombinant Replication Protein A1 (RPA1), Prokaryotic expression, Asp18-Pro403, N-terminal His and GST Tag

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

STABILITY: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Isoelectric Point: 8.5

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 20 mM Tris, 150 mM NaCl ( pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), Other preservative, ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Expiry Date: 12 months

Target Details

Target: RPA1 (Replication Protein A1, 70kDa (RPA1))

Alternative Name: Replication Protein A1

Background: HSSB, REPA1, RF-A, RP-A, RPA70, Replication protein A 70 kDa DNA-binding subunit, Single-stranded DNA-binding protein

Molecular Weight: 72kDa

UniProt: P27694

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA