ROCK1 Protein (AA 1094-1323) (His tag)

Catalog No. ABIN7416292

This Recombinant ROCK1 protein is produced in Escherichia coli (E. coli).

Quick Overview for ROCK1 Protein (AA 1094-1323) (His tag) (ABIN7416292)

Target: ROCK1 (rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), SDS-PAGE (SDS), Immunogen (Imm), Positive Control (PC)

Purity: > 95 %

Product Details

Protein Characteristics: AA 1094-1323

Purification tag / Conjugate: This ROCK1 protein is labelled with His tag.

Purpose: Recombinant Rho Associated Coiled Coil Containing Protein Kinase 1 (Rock1)

Sequence: Leu1094-Gly1323

Characteristics: Recombinant Rho Associated Coiled Coil Containing Protein Kinase 1 (Rock1), Prokaryotic expression, Leu1094-Gly1323, N-terminal His Tag

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

STABILITY: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Isoelectric Point: 7.3

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 20 mM Tris, 150 mM NaCl ( pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Expiry Date: 12 months

Target Details

Target: ROCK1 (rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1))

Alternative Name: Rho Associated Coiled Coil Containing Protein Kinase 1

Background: P160ROCK, Rho-associated protein kinase 1, Renal carcinoma antigen NY-REN-35

Molecular Weight: 33kDa

UniProt: P70335

Pathways: Microtubule Dynamics, WNT Signaling, M Phase, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling