Rhodopsin Protein (RHO) (His tag,GST tag)

Catalog No. ABIN7413442

Recombinant Rhodopsin protein expressed in Escherichia coli (E. coli).

Quick Overview for Rhodopsin Protein (RHO) (His tag,GST tag) (ABIN7413442)

Target: Rhodopsin (RHO)

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Western Blotting (WB), Immunogen (Imm), Positive Control (PC)

Purity: > 80 %

Product Details

Purification tag / Conjugate: This Rhodopsin protein is labelled with His tag,GST tag.

Purpose: Recombinant Rhodopsin (RHO)

Sequence: Met1-Cys110

Characteristics: Recombinant Rhodopsin (RHO), Prokaryotic expression, , N-terminal His-GST Tag

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

STABILITY: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Isoelectric Point: 7.5

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in PBS or others.

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Expiry Date: 12 months

Target Details

Target: Rhodopsin (RHO)

Alternative Name: Rhodopsin

Target Type: Chemical

Background: OPN2, RP4, Opsin 2, Rod Pigment, Retinitis Pigmentosa 4, Visual Purple

Molecular Weight: 42kDa

Pathways: WNT Signaling, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction