IL4 Receptor Protein (AA 26-232) (mFc Tag)

Catalog No. ABIN7092716

This Recombinant IL4 Receptor protein is expressed in HEK-293 Cells.

Quick Overview for IL4 Receptor Protein (AA 26-232) (mFc Tag) (ABIN7092716)

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Screening Assay (ScA), Immunoassay (IA), Antibody Drug Assay (ADA), Affinity Measurement (AM)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 26-232

Purification tag / Conjugate: This IL4 Receptor protein is labelled with mFc Tag.

Purpose: Recombinant Human IL4RA with C-terminal mouse Fc tag

Specificity: IL4RA(Met26-His232) mFc(Pro99-Lys330)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Alternative Name: IL4RA

Background: CD124,IL-4RA,IL4RA
This gene encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE production. The encoded protein also can bind interleukin 4 to promote differentiation of Th2 cells. A soluble form of the encoded protein can be produced by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. Allelic variations in this gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema. Polymorphisms in this gene are also associated with resistance to human immunodeficiency virus type-1 infection. Alternate splicing results in multiple transcript variants.

Molecular Weight: predicted molecular mass of 49.8 kDa after removal of the signal peptide.The apparent molecular mass of IL4RA-mFc is 70-100 kDa due to glycosylation.

UniProt: P24394

Pathways: JAK-STAT Signaling, Positive Regulation of Immune Effector Process