EGFRviii Protein (Fc Tag)

Catalog No. ABIN7199068

Recombinant EGFRviii protein expressed in HEK-293 Cells.

Quick Overview for EGFRviii Protein (Fc Tag) (ABIN7199068)

Target: EGFRviii (EGFRv3) (EGFR vIII (EGFRv3))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: >95 % as determined by SDS-PAGE.

Grade: MALS verified

Product Details

Purification tag / Conjugate: This EGFRviii protein is labelled with Fc Tag.

Purpose: Human EGFRvIII Protein, Fc Tag (MALS verified)

Sequence: Leu 25- Ser 378

Characteristics: Human EGFRvIII, Fc Tag (EGI-H5255) is expressed from human 293 cells (HEK293). It contains AA Leu 25- Ser 378 (Accession # NP_001333870.1).

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Application Notes: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 65.1 kDa. The protein migrates as 90-100 kDa under reducing (R) condition due to glycosylation.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH 7.5

Storage: -20 °C

Storage Comment: -20°C

Target Details

Target: EGFRviii (EGFRv3) (EGFR vIII (EGFRv3))

Alternative Name: EGFRvIII

Background: Synonyms: EGFRvIII,
Description: The epidermal growth factor receptor (EGFR, ErbB-1, HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer. The type III EGF deletion-mutant receptor (EGFRvIII) is the most common mutation and was first identified in primary human glioblastoma tumors, EGFR gene amplification is correlated with the structural rearrangement of the gene. The EGFRvIII gene has an in-frame deletion of 801 base pairs, corresponding to exons 2-7 in the mRNA, resulting in the deletion of amino acids 30-297 in the extracellular domain and the generation of a glycine at the fusion point

Molecular Weight: 65.1 kDa

NCBI Accession: NP_001333870