EGFRviii Protein (AA 25-378) (His-Avi Tag,Biotin)
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- Target See all EGFRviii (EGFRv3) products
- EGFRviii (EGFRv3) (EGFR vIII (EGFRv3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 25-378
- Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This EGFRviii protein is labelled with His-Avi Tag,Biotin.
- Purpose
- Biotinylated Human EGFRVIII Protein
- Sequence
- Leu25-Ser378
- Characteristics
- Recombinant Biotinylated Human EGFRVIII Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu25-Ser378.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Biotinylated Human EGFRVIII, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-EGFRVIII Antibody, hFc Tag with the EC50 of 16.1ng/ml determined by ELISA. See testing image for detail.
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EGFR vIII (EGFRv3) protein (His-Avi Tag)
EGFRv3 Origin: Human Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
EGFR vIII (EGFRv3) (AA 25-378) protein (FITC,His-Avi Tag)EGFRv3 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
EGFR vIII (EGFRv3) (AA 25-378) protein (PE,His-Avi Tag)EGFRv3 Origin: Human Host: HEK-293 Cells Recombinant
EGFR vIII (EGFRv3) (AA 25-378) (Active) protein (His tag,FITC)EGFRv3 Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- EGFRviii (EGFRv3) (EGFR vIII (EGFRv3))
- Alternative Name
- EGFRVIII (EGFRv3 Products)
- Background
- The epidermal growth factor receptor (EGFR) is overexpressed in a variety of human epithelial tumors, often as a consequence of gene amplification. Tumors with EGFR gene amplification frequently contain EGFR gene rearrangements, with the most common extracellular domain mutation being EGFRvIII. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand.
- Molecular Weight
- 41.6 kDa. Due to glycosylation, the protein migrates to 60-78 kDa based on Tris-Bis PAGE result.
- NCBI Accession
- NP_001333870
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