LIF Protein
Quick Overview for LIF Protein (ABIN7199480)
Target
See all LIF ProteinsProtein Type
Origin
Source
Purity
Grade
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Purpose
- ActiveMax® Mouse LIF Protein, Tag Free (MALS verified)
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Sequence
- Ser 24 - Phe 203
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Characteristics
- ActiveMax® Mouse LIF, Tag Free (LIF-M5219) is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Phe 203 (Accession # P09056-1).
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Endotoxin Level
- Less than 1.0 EU per μg by the LAL method.
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Leukemia Inhibitory Factor (LIF) (AA 23-202) protein
LIF Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Leukemia Inhibitory Factor (LIF) (AA 24-203) (Active) proteinLIF Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Leukemia Inhibitory Factor (LIF) (AA 25-203) protein (Fc Tag)LIF Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Leukemia Inhibitory Factor (LIF) (Active) proteinLIF Origin: Rat Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Leukemia Inhibitory Factor (LIF) (AA 23-202) (Active) protein (GST tag)LIF Origin: Human, Mouse Host: Escherichia coli (E. coli) Recombinant >95 % (SDS-PAGE) SDS Active
Leukemia Inhibitory Factor (LIF) (AA 23-202) protein (His tag)LIF Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % by SDS-PAGE gel analyses WB, ELISA
Leukemia Inhibitory Factor (LIF) (AA 24-203) protein (His tag)LIF Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Leukemia Inhibitory Factor (LIF) (AA 1-202) protein (GST tag)LIF Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Leukemia Inhibitory Factor (LIF) proteinLIF Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE
Leukemia Inhibitory Factor (LIF) (AA 23-202) protein (His-GST)LIF Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90% by SDS-PAGE SDS
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Application Notes
- This protein carries no "tag". The protein has a calculated MW of 20.0 kDa. The protein migrates as 35-45 kDa under reducing (R) condition due to glycosylation.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
- PBS, pH 7.4
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Storage
- -20 °C
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Storage Comment
- -20°C
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- LIF (Leukemia Inhibitory Factor (LIF))
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Alternative Name
- LIF
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Background
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Synonyms: LIF,CDF,DIA,HILDA,MLPLI,
Description: Leukemia inhibitory factor, or LIF, an interleukin 6 class cytokine, is a protein in cells that affects cell growth and development.Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. Therefore LIF is used in mouse embryonic stem cell culture. It is necessary to maintain the stem cells in an undifferentiated state, however genetic manipulation of embryonic stem cells allows for LIF independent growth, notably overexpression of the gene Nanog. LIF is not required for culture of human embryonic stem cells. -
Molecular Weight
- 20.0 kDa
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NCBI Accession
- NP_032527
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Pathways
- JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding
Target
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