c-MET Protein (Fc Tag)

Catalog No. ABIN7121559

This Recombinant c-MET protein is expressed in HEK-293 Cells.

Quick Overview for c-MET Protein (Fc Tag) (ABIN7121559)

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Protein Type: Recombinant

Origin: Rhesus Monkey

Source: HEK-293 Cells

Purity: >90 % as determined by SDS-PAGE.

Product Details

Purification tag / Conjugate: This c-MET protein is labelled with Fc Tag.

Purpose: Rhesus macaque HGF R / c-MET Protein,Fc Tag

Sequence: Glu 25 - Asn 930

Characteristics: Rhesus macaque HGF R,Fc Tag (MET-R5256) is expressed from human 293 cells (HEK293). It contains AA Glu 25 - Asn 930 (Accession # G7MM61-1).

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Application Notes: This protein carries a human IgG1 Fc tag at the C-terminus. The mature form of HGF R is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. The protein has a calculated MW of 127.8 kDa (α chain 32.5 kDa and β chain 95.3 kDa). The protein migrates as >116 kDa (alpha & beta chain), 45 kDa (α chain) and (β chain) kDa under reducing (R) condition due to glycosylation.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH 7.5

Storage: -20 °C

Storage Comment: -20°C

Target Details

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Alternative Name: HGF R / c-MET

Background: Synonyms: MET,AUTS9,HGFR,RCCP2,c-Met,
Description: Hepatocyte growth factor receptor (HGFR) is also known as mesenchymal-epithelial transition factor (MET), c-Met, and is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGFR protein possesses tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. HGFR is normally expressed by cells of epithelial origin, while expression of HGF is restricted to cells of mesenchymal origin. Upon HGF stimulation, HGFR induces several biological responses that collectively give rise to a program known as invasive growth. Abnormal HGFR activation in cancer correlates with poor prognosis, where aberrantly active HGFR triggers tumor growth, formation of new blood vessels (angiogenesis) that supply the tumor with nutrients, and cancer spread to other organs (metastasis). HGFR is deregulated in many types of human malignancies, including cancers of kidney, liver, stomach, breast, and brain. Normally, only stem cells and progenitor cells express HGFR, However, cancer stem cells are thought to hijack the ability of normal stem cells to express HGFR, and thus become the cause of cancer persistence and spread to other sites in the body. Various mutations in the HGFR gene are associated with papillary renal carcinoma. HGFR mediates a complex program known as invasive growth. Activation of HGFR triggers mitogenesis, and morphogenesis.

Molecular Weight: 32.5 kDa (α chain) & 95.3 kDa (β chain)

NCBI Accession: NP_001162100

Pathways: RTK Signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor