BRAF Protein (AA 8-170) (His-IF2DI Tag)

Catalog No. ABIN7121967

This Recombinant BRAF protein is expressed in Escherichia coli (E. coli).

Quick Overview for BRAF Protein (AA 8-170) (His-IF2DI Tag) (ABIN7121967)

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), ELISA

Purity: Greater than 90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 8-170

Purification tag / Conjugate: This BRAF protein is labelled with His-IF2DI Tag.

Purpose: Recombinant Human BRAF

Sterility: 0.2 μm filtered

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.

Buffer: Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5 % trehalose, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.

Expiry Date: 12 months

Target Details

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Alternative Name: BRAF

Background: 94 kDa B-raf protein, B-Raf proto-oncogene serine/threonine-protein kinase (p94), BRaf, B-Raf, BRAF1, B-RAF1, BRAF1MGC126806, EC 2.7.11.1, MGC138284, murine sarcoma viral (v-raf) oncogene homolog B1, NS7, p94, Proto-oncogene B-Raf, RAFB1

Molecular Weight: 37.8 kDa

UniProt: P15056

Pathways: MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy