Dihydrofolate Reductase Protein (DHFR) (AA 1-187) (His tag)
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- Target See all Dihydrofolate Reductase (DHFR) Proteins
- Dihydrofolate Reductase (DHFR)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-187
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Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This Dihydrofolate Reductase protein is labelled with His tag.
- Application
- ELISA, Western Blotting (WB)
- Purity
- Greater than 95 % as determined by SDS-PAGE
- Sterility
- 0.2 μm filtered
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Dihydrofolate Reductase (DHFR) (AA 1-187) protein (His tag)
DHFR Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS - PAGE SDS
Dihydrofolate Reductase (DHFR) (AA 1-187) protein (GST tag)DHFR Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Dihydrofolate Reductase (DHFR) (AA 2-187) protein (His-SUMO Tag)DHFR Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Dihydrofolate Reductase (DHFR) protein (Myc-DYKDDDDK Tag)DHFR Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the vial at 10,000 rpm for 1 minute, reconstitute at 200 μg/mL in sterile distilled water by gentle pipetting 2-3 times, don't vortex
- Buffer
- Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5 % trehalose, pH 7.4
- Storage
- 4 °C,-20 °C
- Storage Comment
- -20°C for 12 months as lyophilized, 2-8°C for 1 month under sterile conditions after reconstitution
- Expiry Date
- 12 months
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- Target
- Dihydrofolate Reductase (DHFR)
- Alternative Name
- Dihydrofolate reductase DHFR (DHFR Products)
- Background
- DHFR, DHFRP1, Dihydrofolate reductase, DYR, DYR_HUMAN, EC 1.5.1.3
- Molecular Weight
- 20.5 kDa
- UniProt
- P00375
- Pathways
- Mitotic G1-G1/S Phases
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