IGF1 Protein (AA 49-118) (His tag)
Quick Overview for IGF1 Protein (AA 49-118) (His tag) (ABIN7123450)
Target
See all IGF1 ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 49-118
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Purification tag / Conjugate
- This IGF1 protein is labelled with His tag.
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Purpose
- Recombinant Rat IGF1
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- < 1 EU/μg as determined by LAL test.
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Biological Activity Comment
- Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is 1-3.3 ng/mL.
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Insulin-Like Growth Factor 1 (IGF1) (AA 49-118) (Active) protein
IGF1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Insulin-Like Growth Factor 1 (IGF1) (AA 49-138) protein (GST tag)IGF1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Insulin-Like Growth Factor 1 (IGF1) (AA 1-153) protein (GST tag)IGF1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Insulin-Like Growth Factor 1 (IGF1) (AA 1-115) (Active) proteinIGF1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % pure SDS Active
Insulin-Like Growth Factor 1 (IGF1) (Active) proteinIGF1 Origin: Rat Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Insulin-Like Growth Factor 1 (IGF1) (Active) proteinIGF1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Insulin-Like Growth Factor 1 (IGF1) (AA 49-118) protein (His-IF2DI Tag)IGF1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Insulin-Like Growth Factor 1 (IGF1) protein (Fc Tag)IGF1 Origin: Human Host: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Insulin-Like Growth Factor 1 (IGF1) protein (Fc Tag)IGF1 Origin: Mouse Host: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.
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Buffer
- Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5 % trehalose, pH 7.4.
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Storage
- -20 °C,-80 °C
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Storage Comment
- The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.
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Expiry Date
- 12 months
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- IGF1 (Insulin-Like Growth Factor 1 (IGF1))
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Alternative Name
- IGF1
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Background
- IBP1, IGF I, IGF IA, IGF IB, IGF-I, Igf1, IGF1_HUMAN, IGF1A, IGFI, IGFIA, Insulin like growth factor 1, Insulin like growth factor 1 (somatomedin C)
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Molecular Weight
- 9.6 kDa
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UniProt
- P08025
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Pathways
- RTK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation
Target
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