NCK1 Protein (AA 1-377) (His-IF2DI Tag)

Catalog No. ABIN7124117

This Recombinant NCK1 protein is produced in Escherichia coli (E. coli).

Quick Overview for NCK1 Protein (AA 1-377) (His-IF2DI Tag) (ABIN7124117)

Target: NCK1 (NCK Adaptor Protein 1 (NCK1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), ELISA

Purity: Greater than 90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 1-377

Purification tag / Conjugate: This NCK1 protein is labelled with His-IF2DI Tag.

Purpose: Recombinant Human NCK1

Sterility: 0.2 μm filtered

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.

Buffer: Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5 % trehalose, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.

Expiry Date: 12 months

Target Details

Target: NCK1 (NCK Adaptor Protein 1 (NCK1))

Alternative Name: NCK1

Background: Cytoplasmic protein NCK1, Melanoma Nck protein, MGC12668, NCK, NCK 1, NCK adaptor protein 1, NCK alpha, NCK tyrosine kinase, Nck-1, NCK1, NCK1_HUMAN, NCKalpha, Non catalytic region of tyrosine kinase, SH2/SH3 adaptor protein NCK alpha, SH2/SH3 adaptor protein NCK-alpha

Molecular Weight: 41.4 kDa

UniProt: P16333

Pathways: TCR Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals