IL13 Receptor alpha 1 Protein (Fc-Avi Tag,Biotin)

Catalog No. ABIN7199580

Recombinant IL13 Receptor alpha 1 protein expressed in HEK-293 Cells.

Quick Overview for IL13 Receptor alpha 1 Protein (Fc-Avi Tag,Biotin) (ABIN7199580)

Target: IL13 Receptor alpha 1 (IL13RA1) (Interleukin 13 Receptor, alpha 1 (IL13RA1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: >90 % as determined by SDS-PAGE.

Grade: SPR verified

Product Details

Purification tag / Conjugate: This IL13 Receptor alpha 1 protein is labelled with Fc-Avi Tag,Biotin.

Purpose: Biotinylated Human IL-13 R alpha 1 Protein, Fc,Avitag™ (SPR verified)

Sequence: Gly 22 - Thr 343

Characteristics: Biotinylated Human IL-13 R alpha 1, Fc,Avitag is expressed from human 293 cells (HEK293). It contains AA Gly 22 - Thr 343 (Accession # NP_001551.1).

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Application Notes: This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 65.0 kDa. The protein migrates as 70-90 kDa under reducing (R) condition due to glycosylation.

Comment:

Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH 7.5

Storage: -20 °C

Storage Comment: -20°C

Target Details

Target: IL13 Receptor alpha 1 (IL13RA1) (Interleukin 13 Receptor, alpha 1 (IL13RA1))

Alternative Name: IL-13 R alpha 1

Background: Synonyms: IL13RA1,CD213A1,IL-13Ra,NR4,RP13-128O4.2,IL13Rα1,
Interleukin 13 receptor, alpha 1 is also known as IL13RA1, NR4 and CD213A1 (cluster of differentiation 213A1), The IL13 Rα1 cDNA encodes a 427 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide, a 324 aa residue extracellular domain, a 23 aa residue transmembrane region and a 59 aa residue cytoplasmic tail. Human and mouseIL13Rα1 share 76 % aa sequence identity. IL13RA1 is a subunit of the interleukin 13 receptor. This subunit forms a receptor complex with IL4 receptor alpha, a subunit shared by IL13 and IL4 receptors. This subunit serves as a primary IL13-binding subunit of the IL13 receptor, and may also be a component of IL4 receptors. This protein has been shown to bind tyrosine kinase TYK2, and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3 and STAT6 induced by IL13 and IL4.

Molecular Weight: 65.0 kDa

NCBI Accession: NP_001551

Pathways: JAK-STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response