RAGE Protein

Catalog No. ABIN7317516

This Recombinant RAGE protein is expressed in HEK-293 Cells.

Quick Overview for RAGE Protein (ABIN7317516)

Target: RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Application: ELISA

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purpose: Recombinant Human AGER/RAGE Protein

Sequence: Met 1-Ala 344

Characteristics: A DNA sequence encoding the mature form of human AGER (NP_001127.1) (Met1-Ala 344) was expressed with six amino acids (LEVLFQ) at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: 1. Measured by its ability to compete with Biotinylated recombinant human AGER for binding to immobilized recombinant human Fc-S100B in a functional ELISA. 2. Measured by its ability to compete with Biotinylated recombinant human AGER for binding to immobilized recombinant mouse S100B-Fc in a functional ELISA. 3. Measured by its ability to compete with Biotinylated recombinant human AGER for binding to immobilized recombinant human S100A1-Fc in a functional ELISA. 4. Measured by its ability to compete with Biotinylated recombinant human AGER for binding to immobilized recombinant human APP-Fc in a functional ELISA.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))

Alternative Name: AGER/RAGE

Background: AGER,Advanced Glycosylation End Product-Specific Receptor,RAGE,Receptor for Advanced Glycosylation End Products,Receptor for Advanced Glycosylation End Products (RAGE, or AGER) is a member of the immunoglobulin super-family transmembrane proteins, as a signal transduction receptor which binds advanced glycation endproducts, certain members of the S100/calgranulin family of proteins, high mobility group box 1 (HMGB1), advanced oxidation protein products, and amyloid (beta-sheet fibrils). Initial studies investigating the role of RAGE in renal dysfunction focused on diabetes, neurodegenerative disorders, and inflammatory responses. However, RAGE also has roles in the pathogenesis of renal disorders that are not associated with diabetes, such as obesity-related glomerulopathy, doxorubicin-induced nephropathy, hypertensive nephropathy, lupus nephritis, renal amyloidosis, and ischemic renal injuries. RAGE represents an important factor in innate immunity against pathogens, but it also interacts with endogenous ligands, resulting in chronic inflammation. RAGE signaling has been implicated in multiple human illnesses, including atherosclerosis, arthritis, Alzheimer's disease, atherosclerosis and aging associated diseases.

Molecular Weight:

Calculated MW: 35.0 kDa

Observed MW: 46-52 kDa

NCBI Accession: NP_001127

Pathways: Carbohydrate Homeostasis, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, S100 Proteins