BDNF Protein

Catalog No. ABIN7319738

This Recombinant BDNF protein is produced in Escherichia coli (E. coli).

Quick Overview for BDNF Protein (ABIN7319738)

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human, Rat, Mouse

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purpose: Recombinant Human/Mouse/Rat BDNF Protein (Active)

Sequence: His129-Arg247

Characteristics: Recombinant Human/Mouse/Rat BDNF is produced by our E.coli expression system and the target gene encoding His129-Arg247 is expressed.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized Human BDNF at 2ug/mll(100 μl/well) can bind Human TrkB-His(Cat: PKSH033579). The ED50 of Human BDNF is 2.82 ug/ml .

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 250 mM NaCl, pH 7.2.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Alternative Name: BDNF

Background:

Background: Brain-Derived Neurotrophic Factor (BDNF) is a member of the neurotrophin family. Along with other structurally related neurotrophic factors NGF, NT-3 and NT-4, BDNF binds with high affinity to the TrkB kinase receptor. It also binds with the LNGFR (for low-affinity nerve growth factor receptor, also known as p75). BDNF promotes the survival, growth and differentiation of neurons. It serves as a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. BDNF expression is altered in neurodegenerative disorders such as Parkinson',s and Alzheimer',s disease.

Synonym: Brain-Derived Neurotrophic Factor, BDNF, Abrineurin

Molecular Weight: 13 kDa

UniProt: P23560

Pathways: RTK Signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity