CPB1 Protein (His tag)

Catalog No. ABIN7194606

This Recombinant CPB1 protein is produced in HEK-293 Cells.

Quick Overview for CPB1 Protein (His tag) (ABIN7194606)

Target: CPB1 (Carboxypeptidase B1 (Tissue) (CPB1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 94 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This CPB1 protein is labelled with His tag.

Purpose: Recombinant Mouse Carboxypeptidase B1/CPB1 Protein (His Tag)(Active)

Sequence: Met 1-Tyr 415

Characteristics: A DNA sequence encoding the mouse CPB1 (NP_083982.1) precursor (Met 1-Tyr 415) was expressed, with a C-terminal polyhistidine tag.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to cleave a colorimetric peptide substrate, Hippuryl-Arg. The specific activity is >5,000 pmoles/min/μg.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM MES, 150 mM NaCl, pH 6.5

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CPB1 (Carboxypeptidase B1 (Tissue) (CPB1))

Alternative Name: Carboxypeptidase B1/CPB1

Background:

Background: Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 307-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.

Synonym: 0910001A18Rik,1810063F02Rik,2210008M23Rik,AI504870

Molecular Weight: 47.5 kDa

NCBI Accession: NP_083982