CPB1 Protein (His tag)

Catalog No. ABIN7194604

Product Details

Target: CPB1 (Carboxypeptidase B1 (Tissue) (CPB1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CPB1 protein is labelled with His tag.

Purpose: Recombinant Human Carboxypeptidase B1/CPB1 Protein (His Tag)(Active)

Sequence: Met 1-Tyr 417

Characteristics: A DNA sequence encoding the human CPB1 (NP_001862.2) (Met 1-Tyr 417) was expressed with a C-terminal polyhistidine tag.

Purity: > 98 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its ability to cleave a colorimetric peptide substrate, Hippuryl-Arg, as measured using the wavelength at 254 nm.The specific activity is >10000 pmoles/min/μg.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 25 mM MES, 0.1 M NaCl, pH 6.5

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CPB1 (Carboxypeptidase B1 (Tissue) (CPB1))

Alternative Name: Carboxypeptidase B1/CPB1 (CPB1 Products)

Synonyms: wu:fb60d07 Protein, wu:fb69b08 Protein, CPB1 Protein, pCPB Protein, CPB Protein, PASP Protein, PCPB Protein, Cpb Protein, 0910001A18Rik Protein, 1810063F02Rik Protein, 2210008M23Rik Protein, AI504870 Protein, carboxypeptidase B1 (tissue) Protein, carboxypeptidase B1 Protein, carboxypeptidase B1 S homeolog Protein, carboxypeptidase B Protein, cpb1 Protein, CPB1 Protein, cpb1.S Protein, CpipJ_CPIJ010799 Protein, CpipJ_CPIJ010801 Protein, VDBG_02663 Protein, LOC100562609 Protein, Cpb1 Protein

Background:

Background: Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 307-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.

Synonym: Carboxypeptidase B, Pancreas-Specific Protein, PASP, CPB1, CPB, PCPB

Molecular Weight: 47 kDa

NCBI Accession: NP_001862