CARTPT Protein (mFc Tag)

Catalog No. ABIN7317277

Recombinant CARTPT protein expressed in HEK-293 Cells.

Quick Overview for CARTPT Protein (mFc Tag) (ABIN7317277)

Target: CARTPT (CART Prepropeptide (CARTPT))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This CARTPT protein is labelled with mFc Tag.

Purpose: Recombinant Human CART/CARTPT Protein (Fc Tag)

Sequence: Met 1-Leu116

Characteristics: A DNA sequence encoding the human CARTPT (NP_004282.1) (Met1-Leu116) was expressed with the Fc region of mouse IgG1 at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: CARTPT (CART Prepropeptide (CARTPT))

Alternative Name: CART/CARTPT

Background: CART,In RNAseq data from human islet donors, CARTPT expression levels correlated with insulin, exocytosis genes and key beta-cell transcription factors.  Insufficient insulin release and hyperglucagonaemia are culprits in type 2 diabetes. Cocaine- and amphetamine-regulated transcript (CART, encoded by Cartpt) affects islet hormone secretion and beta cell survival in vitro in rats, and Cart (-/-) mice have diminished insulin secretion. Established a potential role for cocaine and amphetamine regulated transcript (CARTPT) in dominant follicle selection in cattle. CARTPT expression is elevated in subordinate versus dominant follicles, and treatment with the mature form of the CARTPT peptide (CART) decreases follicle-stimulating hormone (FSH)-stimulated granulosa cell estradiol production in vitro and follicular fluid estradiol and granulosa cell CYP19A1 mRNA in vivo.

Molecular Weight:

Calculated MW: 36.3 kDa

Observed MW: 37 kDa

Gene ID: 9607

NCBI Accession: NP_004282

UniProt: Q16568

Pathways: Hormone Transport, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Feeding Behaviour