CTSA Protein (His tag)

Catalog No. ABIN7194664

This Recombinant CTSA protein is expressed in HEK-293 Cells.

Quick Overview for CTSA Protein (His tag) (ABIN7194664)

Target: CTSA (Cathepsin A (CTSA))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This CTSA protein is labelled with His tag.

Purpose: Recombinant Human Cathepsin A/CTSA Protein (His Tag)

Sequence: Met 1-Tyr 480

Characteristics: A DNA sequence encoding the human cathepsin A isoform b (Met 1-Tyr 480) (NP_001121167.1) was expressed with a N-terminal signal peptide and a C-terminal polyhistidine tag.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 25 mM Tris, 0.15 mM NaCl, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: CTSA (Cathepsin A (CTSA))

Alternative Name: Cathepsin A/CTSA

Background: CTSA,Carboxypeptidase C,Carboxypeptidase L,Cathepsin A,GLB2,GSL,Lysosomal protective protein,NGBE,PPCA,PPGB,Lysosomal carboxypeptidase, cathepsin A (protective protein, CathA), is a component of the lysosomal multienzyme complex along with beta-galactosidase (GAL) and sialidase Neu1, where it activates Neu1 and protects GAL and Neu1 against the rapid proteolytic degradation. Cathepsin A is a multicatalytic enzyme with deamidase and esterase in addition to carboxypeptidase activities. It was recently identified in human platelets as deamidase. In vitro, it hydrolyzes a variety of bioactive peptide hormones including tachykinins, suggesting that extralysosomal cathepsin A plays a role in regulation of bioactive peptide functions. It is a member of the alpha/beta hydrolase fold family and has been suggested to share a common ancestral relationship with other alpha/beta hydrolase fold enzymes, such as cholinesterases. Cathepsin A defects are linked to multiple forms of Galactosialidosis with a combined secondary deficiency of beta-galactosidase and neuraminidase. Cathepsin A is a key molecule in the onset of galactosialidosis and also highlight the therapeutic acts in vivo as an endothelin-1-inactivating enzyme and strongly confirm a crucial role of this enzyme in effective elastic fiber formation.

Molecular Weight:

Calculated MW: 53.0 kDa

Observed MW: 53 kDa

Gene ID: 5476

NCBI Accession: NP_001121167

UniProt: P10619