CD36 Protein (CD36) (His-Fc tag)

Catalog No. ABIN7320283

Recombinant CD36 protein expressed in HEK-293 Cells.

Quick Overview for CD36 Protein (CD36) (His-Fc tag) (ABIN7320283)

Target: CD36

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Application: ELISA

Purity: > 88 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This CD36 protein is labelled with His-Fc tag.

Purpose: Recombinant Mouse CD36/SCARB3 Protein (His & Fc Tag)

Sequence: Gly 30-Lys 439

Characteristics: A DNA sequence encoding the extracellular domain of mouse CD36 (NP_001153030.1) (Gly 30-Lys 439) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Immobilized human RSPO1 at 20 μg/ml (100 μl/well) can bind mouse CD36 Fc chimera with a linear ranger of 6. 4-800 ng/ml.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: CD36

Alternative Name: CD36/SCARB3

Background: FAT,GPIV,Scarb3,The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 36 (CD36), also known as FAT, SCARB3, GP88, glycoprotein IV (gpIV) and glycoprotein IIIb (gpIIIb), is a member of the CD system as well as the class B scavenger receptor family of cell surface proteins. CD36 can be found on the surface of many cell types in vertebrate animals and it consists of 472 amino acids and is extensively glycosylated. It is an integral membrane protein primarily serving as receptors for thrombospondin and collagen and by the erythrocytes infected with the human malaria parasite. The role of CD36 as a cell surface receptor has been extended to that of a signal transduction molecule.

Molecular Weight:

Calculated MW: 74.5 kDa

Observed MW: 110-120 kDa

Gene ID: 12491

NCBI Accession: NP_001153030

UniProt: Q08857

Pathways: TLR Signaling, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Hepatitis C, Toll-Like Receptors Cascades, Lipid Metabolism, S100 Proteins