CD40 Ligand Protein (CD40LG)

Catalog No. ABIN7318281

This Recombinant CD40 Ligand protein is expressed in HEK-293 Cells.

Quick Overview for CD40 Ligand Protein (CD40LG) (ABIN7318281)

Target: CD40 Ligand (CD40LG)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Application: Bio-Layer Interferometry (BLI)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purpose: Recombinant Human CD40L/TNFSF5 Protein (Human Cells)

Sequence: Met 113-Leu261

Characteristics: Recombinant Human CD40 Ligand is produced by our Mammalian expression system and the target gene encoding Met113-Leu261 is expressed.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Loaded Human CD40-Fc on Protein A Biosensor, can bind Human CD40L with an affinity constant of 3.29 nM as determined in BLI assay.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 200 mM NaCl, 0.1 mM EDTA, pH 7.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: CD40 Ligand (CD40LG)

Alternative Name: CD40L/TNFSF5

Background: CD154,CD40 Ligand,CD40-L,CD40L,CD40LG,HIGM1,IGM,IMD3,T-BAM,T-Cell Antigen Gp39,TNF-Related Activation Protein,TNFSF5,TRAP,Tumor Necrosis Factor Ligand Superfamily Member 5,hCD40L,CD40 Ligand (CD40LG) is a type II transmembrane glycoprotein that belongs to the TNF superfamily. Like other TNF superfamily members, CD40LG exists as a trimer in membrane bound and soluble form, both of which are bioactive. CD40LG is a ligand for CD40, its ligation also initiates signal transduction in CD40LG expressing cells. CD40LG is a differentiation antigen that is expressed on the surface of T-cells. It stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40LG has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL2.

Molecular Weight:

Calculated MW: 16.2 kDa

Observed MW: 16-18 kDa

Gene ID: 959

UniProt: P29965

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints