CD58 Protein (CD58) (Fc Tag)

Catalog No. ABIN7317383

This Recombinant CD58 protein is expressed in HEK-293 Cells.

Quick Overview for CD58 Protein (CD58) (Fc Tag) (ABIN7317383)

Target: CD58

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Application: ELISA

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This CD58 protein is labelled with Fc Tag.

Purpose: Recombinant Human LFA-3/CD58 Protein (Fc Tag)

Sequence: Met 1-Arg215

Characteristics: A DNA sequence encoding the human CD58 (Q9BRW0) (Met1-Arg215) was expressed with the Fc region of human IgG1 at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: 1. Immobilized human CD2-His at 10 μg/ml (100 μl/well) can bind human CD58-Fc, The EC₅₀ of human CD58-Fc is 0.04-0.1 μg/ml. 2. Immobilized Cynomolgus CD2-His at 10 μg/ml (100 μl/well) can bind human CD58-Fc, The EC₅₀ of human CD58-Fc is 0.04-0.10 μg/ml.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: CD58

Alternative Name: LFA-3/CD58

Background: CD295,DB,HuB219,LEP-R,LEPR,Leptin receptor,OB receptor,OB-R,OBR,CD53 is a member of the transmembrane 4 superfamily, also called the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. These proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD53 is a cell surface glycoprotein that is known to complex with integrins. Familial deficiency of CD53 gene has been linked to an immunodeficiency associated with recurrent infectious diseases caused by bacteria, fungi and viruses. CD53 contributes to the transduction of CD2-generated signals in T cells and natural killer cells and has been suggested to play a role in growth regulation.

Molecular Weight:

Calculated MW: 48.5 kDa

Observed MW: 68 kDa

UniProt: Q9BRW0