CD82 Protein (CD82) (AA 103-225) (Fc Tag)

Catalog No. ABIN7319825

Recombinant CD82 protein expressed in HEK-293 Cells.

Quick Overview for CD82 Protein (CD82) (AA 103-225) (Fc Tag) (ABIN7319825)

Target: CD82

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 103-225

Purification tag / Conjugate: This CD82 protein is labelled with Fc Tag.

Purpose: Recombinant Human CD82 (N-Fc)

Sequence: Gly103-Gln225

Characteristics: Recombinant Human CD82 Antigen is produced by our Mammalian expression system and the target gene encoding Gly103-Gln225 is expressed with a Fc tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: CD82

Alternative Name: CD82

Background: CD82,CD82 antigenC33,CD82 Molecule,IA4,IA4C33 antigen,Inducible membrane protein R2,KAI1,KAI1GR15,ST6,ST6tspan-27,Suppressor of tumorigenicity 6 protein,TSPAN27,TSPAN274F9,Tetraspanin-27,Tspan-27,CD82 antigen, also known as Kai-1, is a widely expressed palmitoylated molecule of the tetraspanin superfamily. KAI1/CD82 is localized on cell membrane and form interactions with other tetraspanins, integrins and chemokines which are respectively responsible for cell migration, adhesion and signaling. CD82/Kai-1 is a component of the promiscuous TIMP-1 interacting protein complex on the cell surface of human adenocarcinoma cells and gives insight into tumorigenic metastatic potential. CD82/Kai-1 suppresses EMT in prostate cancer cells adhered to fibronectin leading to reduced cell migration and invasiveness. CD82/Kai-1 function is important for muscle stem cell function in muscular disorders. Overexpression of CD82/Kai-1 suppresses growth, migration and invasion of oral cancer cells and may be considered as a potential therapeutic target in oral cancer.

Molecular Weight:

Calculated MW: 40.3 kDa

Observed MW: 40-60 kDa

Gene ID: 3732

UniProt: P27701

Pathways: p53 Signaling